Talk:CH391L/S14/GFP

Talk:CH391L/S14/GFP

• Jeffrey Barrick (talk) 16:19, 10 February 2014 (CST) Could you add a table summarizing what's different in common GFP variants? (sfGFP, eGFP, P7, even RFP, CFP, etc.) There's a lot of terminology out there, and it's important for anyone using GFP to choose the right variant.
  • --Mindy (talk) 10:18, 17 February 2014 (CST)I think the table addresses at least the most common variants now.

• --drewtack (talk) 16:46, 10 February 2014 (CST)You might want to include some mention of split gfp. Which is quite popular now days.
  • --Mindy (talk) 10:18, 17 February 2014 (CST)I had mentioned it in the iGEM portion but hadn't used the term- I went back and clarified it now.

• --Dennis Mishler (talk) 14:17, 13 February 2014 (CST) I agree with Ella's comment below about "general diction". In a few places, the word choice could be more formal. For example, "has become an invaluable asset to a great deal of research in the life sciences". I would either remove or change "a great deal" to something more formal.
  • --Mindy (talk) 10:18, 17 February 2014 (CST)Ella also addressed some grammatical issues, I think I resolved all of the ones I could find.

• --Dennis Mishler (talk) 14:17, 13 February 2014 (CST) First figure needs a citation or link.
  • --Mindy (talk) 10:18, 17 February 2014 (CST)Now the link should work, I think I must've deleted it on accident the first time.

• --Dennis Mishler (talk) 14:17, 13 February 2014 (CST) The "mechanisms of chromophore activation" figure could use a more detailed legend/explanation of what the reader is looking at.
  • --Mindy (talk) 10:18, 17 February 2014 (CST)I added some more text, hopefully it's more clear now.

• --Jordan Monk (talk) 03:02, 14 February 2014 (CST) Liked your presentation and your wiki. One minor thing I can add that others haven't mentioned is that Shimomura's first name is Osamu. Also, are you adding the links out to other websites? I had a link just magically appear on my wiki. If it's something you're adding, I think the formatting could be improved.
  • --Mindy (talk) 10:18, 17 February 2014 (CST) I fixed the parentheses, I think that makes it a bit easier to read, like Ella mentioned.

• --Gabriel Suarez (talk) 07:09, 14 February 2014 (CST) I think you should mention somewhere, maybe the intro, why using a GFP reporter tag is not expected to affect normal protein function or if it would. Your wiki report is blessed with cool images! :)
  • --Mindy (talk) 10:18, 17 February 2014 (CST)I put that in the beginning now, good idea.

• --Chen-Hsun Tsai (talk) 08:57, 14 February 2014 (CST) This wiki page is well structured and contain many detail, which is good. I think one more thing you can add is how GFP compared with other fluorescent proteins, like YFP. What's the pros and cons for using GFP rather then other similar proteins?
  • --Mindy (talk) 10:18, 17 February 2014 (CST)I think now that there is a table, that's a bit more clear.

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--Ella Watkins (talk) 13:22, 12 February 2014 (CST): --Mindy (talk) 10:20, 17 February 2014 (CST)Thank you so much for all the advice, I think I've fixed all of the issues mentioned :) Overall Format and structure:

General diction commentary:

• Green Fluorescent Protein (GFP) is a small 27 kD, 238 Ribbon structure of GFP (blue) and chromophore (green) (Conncoll.edu)

amino acid protein, that was discovered in the jellyfish Aequorea victoria. It has since become an valuable asset those in the field of research in the life sciences.

• “when inserted in line downstream of a gene” can be changed “when inserted downstream from a gene”

• If possible, remove parenthesis around (quantum yield), (TRITC), (review article), (review) and any other words that should be in the sentences and hyperlinked. These are throughout your page and I think it will make the page easier to read when the parenthesis are removed.

• “Suggested mechanisms of chromophore activation are displayed at right” maybe change that to “in figure displayed to the right”

• “This is important because it means since no cofactors” take out “since”

I really appreciated the figures you chose, they were very tactfully done! Also I really loved the way that this Wiki was lain out. Very comprehensive, it had a very nice flow from one section to another.

Introduction and background material: Other than a few things that I suggested in my general diction commentary, very well done: There is a brief introduction on what it is, why it is important, and potential applications. I enjoyed the clear and comprehensive overview of what I will be reading in the following sections of the wiki (I honestly think all wikis should start like yours! A very excellently done introduction)

Methods and main body/concepts: The examples of the applications are concise and clear. A suggestion would be to add an example of experiments that used each application. Also, another suggestion would be to elaborate on the Fluorescent tagging section, I thought in your presentation you had a great image of a red/green tagged organism that showed how the proteins were tracked over time… maybe consider adding that photo in?

• • --Mindy (talk) 10:33, 17 February 2014 (CST)I made sure all of the references were papers that used the different techniques, so the citations links out to appropriate experiments.

Relation to iGEM and future directions: Excellent, there is a clear connection to how iGEM utilizes the GFP, as well as a good background of historical information. No editing needed.

• ----Ajv684 (talk) 18:12, 13 February 2014 (CST)Excellent wiki and presentation Mindy, I think you did a great job. It is a very comprehensive approach of the topic. I like the structure of the wiki since you gave a very sound introduction with even a simple-to-understand brief history, then go on the chemistry of the protein and finally to the specific applications, all of them very relevant to synbio. My only suggestion would be add a brief summary of two sentences at the beginning of your wiki so that everybody who reads it knows right away what GFP is and what to expect in reading your wiki.