Difference between revisions of "DNA Assembly Methods(Microbes as Machines)"
From SynBioCyc
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#Gibson2009 pmid=19363495 | #Gibson2009 pmid=19363495 | ||
− | // Describes isothermal DNA assembly (aka Gibson Cloning) | + | // Describes isothermal DNA assembly (aka Gibson Cloning). Additional information on the [https://www.addgene.org/plasmid-protocols/gibson-assembly/ AddGene website]. |
</biblio> | </biblio> | ||
Revision as of 01:58, 9 February 2015
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Required Reading
Error fetching PMID 19363495:
- Error fetching PMID 19363495:
Describes isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website.
Assigned Papers
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [2].
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Error fetching PMID 23360965:
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Construction of synthetic Mycoplasma laboratorium genome - Error fetching PMID 23360965:
Describes CRISPR-based genome editing of Escherichia coli