Difference between revisions of "DNA Assembly Methods(Microbes as Machines)"
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Revision as of 19:31, 8 February 2015
Download Pre-Discussion Questions (PDF)
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process.[2].
Required Reading
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Describes isothermal DNA assembly (aka Gibson Cloning)
Assigned Papers
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Construction of synthetic M. mycoides genome // Describes CRISPR-based genome editing