Difference between revisions of "DNA Assembly Methods(Microbes as Machines)"

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Revision as of 19:31, 8 February 2015

Prototypical 'datasheet' [1].

Download Pre-Discussion Questions (PDF)

The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process.[2].

Required Reading

Error fetching PMID 19363495:
  1. Error fetching PMID 19363495: [Gibson2009]
    Describes isothermal DNA assembly (aka Gibson Cloning)

Assigned Papers

Error fetching PMID 20488990:
  1. Error fetching PMID 20488990: [Gibson2010]
    Construction of synthetic M. mycoides genome

    // Describes CRISPR-based genome editing