Difference between revisions of "DNA Assembly Methods(Microbes as Machines)"
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==== [[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]] ==== | ==== [[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]] ==== | ||
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= Required Reading = | = Required Reading = | ||
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// Describes isothermal DNA assembly (aka Gibson Cloning) | // Describes isothermal DNA assembly (aka Gibson Cloning) | ||
</biblio> | </biblio> | ||
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| + | [[File:Synthia_Genome.png|300px|thumb|right|Construction of the synthetic genome of ''Mycoplasma laboratorium'' <cite>Gibson2010</cite>.]] | ||
= Assigned Papers = | = Assigned Papers = | ||
| + | <blockquote> | ||
| + | <font size="4">The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process <cite>Gibson2010</cite>.</font> | ||
| + | </blockquote> | ||
<biblio> | <biblio> | ||
#Gibson2010 pmid=20488990 | #Gibson2010 pmid=20488990 | ||
Revision as of 19:41, 8 February 2015
Download Pre-Discussion Questions (PDF)
Required Reading
Error fetching PMID 19363495:
- Error fetching PMID 19363495:
Describes isothermal DNA assembly (aka Gibson Cloning)
Assigned Papers
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [2].
Error fetching PMID 20488990:
Error fetching PMID 23360965:
Error fetching PMID 23360965:
- Error fetching PMID 20488990:
Construction of synthetic Mycoplasma laboratorium genome - Error fetching PMID 23360965:
Describes CRISPR-based genome editing of Escherichia coli
