Difference between revisions of "DNA Assembly Methods(Microbes as Machines)"

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==== [[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]] ====
 
==== [[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]] ====
 
= Required Reading =
 
 
<biblio>
 
#Gibson2009 pmid=19363495
 
// Describes isothermal DNA assembly (aka Gibson Cloning). Additional information on the [https://www.addgene.org/plasmid-protocols/gibson-assembly/ AddGene website].
 
</biblio>
 
  
 
[[File:Synthia_Genome.png|300px|thumb|right|Construction of the synthetic genome of ''Mycoplasma laboratorium'' <cite>Gibson2010</cite>.]]  
 
[[File:Synthia_Genome.png|300px|thumb|right|Construction of the synthetic genome of ''Mycoplasma laboratorium'' <cite>Gibson2010</cite>.]]  
  
= Assigned Papers =
 
 
<blockquote>
 
<blockquote>
 
<font size="4">The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process <cite>Gibson2010</cite>.</font>
 
<font size="4">The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process <cite>Gibson2010</cite>.</font>
 
</blockquote>
 
</blockquote>
 +
 +
= Required Reading/Viewing =
 +
 
<biblio>
 
<biblio>
 
#Gibson2010 pmid=20488990
 
#Gibson2010 pmid=20488990
// Construction of synthetic ''Mycoplasma laboratorium'' genome
+
// Construction of synthetic ''Mycoplasma laboratorium'' genome.
#Jiang2013 pmid=23360965
+
#Venter2010 [http://www.ted.com/talks/craig_venter_unveils_synthetic_life?language=en Venter press conference / TED Talk discussing this paper]
// Describes CRISPR-based genome editing of ''Escherichia coli''
+
</biblio>
 +
 
 +
= Assigned Papers =
 +
 
 +
<biblio>
 +
#Hughes2011 pmid=21601682
 +
// Pligonucleotide and gene synthesis methods
 +
#Gibson2009 pmid=19363495
 +
// Isothermal DNA assembly (aka Gibson Cloning). Additional information on the [https://www.addgene.org/plasmid-protocols/gibson-assembly/ AddGene website].
 +
#Kuijpers2013 pmid=24028550
 +
// In vivo DNA assembly using homologous recombination in yeast.
 
</biblio>
 
</biblio>

Revision as of 03:37, 9 February 2015

Download Pre-Discussion Questions (PDF)

Construction of the synthetic genome of Mycoplasma laboratorium [1].
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [1].

Required Reading/Viewing

Error fetching PMID 20488990:
  1. Error fetching PMID 20488990: [Gibson2010]
    Construction of synthetic Mycoplasma laboratorium genome.
  2. Venter press conference / TED Talk discussing this paper [Venter2010]

Assigned Papers

Error fetching PMID 21601682:
Error fetching PMID 19363495:
Error fetching PMID 24028550:
  1. Error fetching PMID 21601682: [Hughes2011]
    Pligonucleotide and gene synthesis methods
  2. Error fetching PMID 19363495: [Gibson2009]
    Isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website.
  3. Error fetching PMID 24028550: [Kuijpers2013]
    In vivo DNA assembly using homologous recombination in yeast.
All Medline abstracts: PubMed | HubMed