Difference between revisions of "DNA Assembly Methods(Microbes as Machines)"

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<biblio>
 
<biblio>
 
#Hughes2011 pmid=21601682
 
#Hughes2011 pmid=21601682
// Pligonucleotide and gene synthesis methods
+
// Oligonucleotide and gene synthesis methods
 
#Gibson2009 pmid=19363495
 
#Gibson2009 pmid=19363495
 
// Isothermal DNA assembly (aka Gibson Cloning). Additional information on the [https://www.addgene.org/plasmid-protocols/gibson-assembly/ AddGene website].
 
// Isothermal DNA assembly (aka Gibson Cloning). Additional information on the [https://www.addgene.org/plasmid-protocols/gibson-assembly/ AddGene website].

Revision as of 03:38, 9 February 2015

Download Pre-Discussion Questions (PDF)

Construction of the synthetic genome of Mycoplasma laboratorium [1].
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [1].

Required Reading/Viewing

Error fetching PMID 20488990:
  1. Error fetching PMID 20488990: [Gibson2010]
    Construction of synthetic Mycoplasma laboratorium genome.
  2. Venter press conference / TED Talk discussing this paper [Venter2010]

Assigned Papers

Error fetching PMID 21601682:
Error fetching PMID 19363495:
Error fetching PMID 24028550:
  1. Error fetching PMID 21601682: [Hughes2011]
    Oligonucleotide and gene synthesis methods
  2. Error fetching PMID 19363495: [Gibson2009]
    Isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website.
  3. Error fetching PMID 24028550: [Kuijpers2013]
    In vivo DNA assembly using homologous recombination in yeast.
All Medline abstracts: PubMed | HubMed