Difference between revisions of "DNA Assembly Methods(Microbes as Machines)"
From SynBioCyc
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#Hughes2011 pmid=21601682 | #Hughes2011 pmid=21601682 | ||
// Oligonucleotide and gene synthesis methods | // Oligonucleotide and gene synthesis methods | ||
| − | # | + | #Gibson2009a pmid=19363495 |
// Isothermal DNA assembly (aka Gibson Cloning). Additional information on the [https://www.addgene.org/plasmid-protocols/gibson-assembly/ AddGene website]. | // Isothermal DNA assembly (aka Gibson Cloning). Additional information on the [https://www.addgene.org/plasmid-protocols/gibson-assembly/ AddGene website]. | ||
#Kuijpers2013 pmid=24028550 | #Kuijpers2013 pmid=24028550 | ||
| − | // In vivo DNA assembly using homologous recombination in yeast | + | // 1/2 In vivo DNA assembly using homologous recombination in yeast |
| + | #Gibson2009b pmid=19745056 | ||
| + | // 2/2 In vivo assembly of single-stranded oligonucleotides in yeast | ||
</biblio> | </biblio> | ||
Revision as of 03:54, 9 February 2015
Download Pre-Discussion Questions (PDF)
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [1].
Required Reading/Viewing
Error fetching PMID 20488990:
- Error fetching PMID 20488990:
Construction of synthetic Mycoplasma laboratorium genome. - Venter press conference / TED Talk discussing this paper
Assigned Papers
Error fetching PMID 21601682:
Error fetching PMID 19363495:
Error fetching PMID 24028550:
Error fetching PMID 19745056:
Error fetching PMID 19363495:
Error fetching PMID 24028550:
Error fetching PMID 19745056:
- Error fetching PMID 21601682:
Oligonucleotide and gene synthesis methods - Error fetching PMID 19363495:
Isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website. - Error fetching PMID 24028550:
1/2 In vivo DNA assembly using homologous recombination in yeast - Error fetching PMID 19745056:
2/2 In vivo assembly of single-stranded oligonucleotides in yeast
