Difference between revisions of "DNA Assembly Methods(Microbes as Machines)"

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== Additional Information ==
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= Additional Information =
  
 
[[File:Synthia_Genome_Assembly_Flight.jpeg|thumbnail|500px|left|Image from [https://cmcagustinos.wordpress.com/2011/02/07/genoma-sintetico-y-celula-artificial/ Genoma sintético y célula artificial]]]
 
[[File:Synthia_Genome_Assembly_Flight.jpeg|thumbnail|500px|left|Image from [https://cmcagustinos.wordpress.com/2011/02/07/genoma-sintetico-y-celula-artificial/ Genoma sintético y célula artificial]]]
  
 
[[File:Venter_Defragmented_Minimal_Genome_Slide.png|thumbnail|500px|left|Image from [https://twitter.com/hashtag/sbbsf14 Image from #SBBSF14]]]
 
[[File:Venter_Defragmented_Minimal_Genome_Slide.png|thumbnail|500px|left|Image from [https://twitter.com/hashtag/sbbsf14 Image from #SBBSF14]]]

Latest revision as of 18:46, 13 February 2015

Contents

Download Pre-Discussion Questions (PDF)

Construction of the synthetic genome of Mycoplasma laboratorium [1].
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [1].

Required Reading/Viewing

Error fetching PMID 20488990:
  1. Error fetching PMID 20488990: [Gibson2010]
    Construction of synthetic Mycoplasma laboratorium genome.
  2. Venter press conference / TED Talk discussing this paper [Venter2010]

Assigned Papers

Error fetching PMID 21601682:
Error fetching PMID 19363495:
Error fetching PMID 24028550:
Error fetching PMID 19745056:
  1. Error fetching PMID 21601682: [Hughes2011]
    Oligonucleotide and gene synthesis methods
  2. Error fetching PMID 19363495: [Gibson2009a]
    Isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website.
  3. Error fetching PMID 24028550: [Kuijpers2013]
    1/2 In vivo DNA assembly using homologous recombination in yeast
  4. Error fetching PMID 19745056: [Gibson2009b]
    2/2 In vivo assembly of single-stranded oligonucleotides in yeast
All Medline abstracts: PubMed | HubMed

Additional Information