Talk:CH391L/S14/CRISPR
- --Dennis Mishler (talk) 08:38, 1 April 2014 (CDT) Gabo, you have a ton of information, which is great. My concern though is in the ordering of that information...
Here is something you e-mailed me: "The CRISPR/Cas system is an RNA-guided endonuclease system found naturally in many prokaryotes. CRISPR stands for Clustered Regularly Inter-Spaced Palindromic Repeats. This small genomic region (less than 1kb) is a critical part of the bacterial defense system against foreign DNA, mainly because it is known to retain genetic information about previous encounters with phage or plasmid DNA. Cas genes, are CRISPR associated genes that encode for several proteins with roles in the formation of the RNA-guided endonuclease complex."
Maybe you needed to change this paragraph a bit, but this was a very good introduction/starting description. I would add a paragraph like this before you go into the background/details. Originally, my one big request from this starting paragraph was to add a one-sentence simple explanation of what CRISPR does... (relating to bacterial defenses). When introducing material, you should start with the bigger/general picture, and then focus on the details.
- --Dennis Mishler (talk) 08:38, 1 April 2014 (CDT) Assuming you add an introduction, I would change the current "background" section to something indicating a comparison of different nuclease-mediated genome editing. This could (perhaps) be the second or third section, after the introduction.
- --Dennis Mishler (talk) 08:38, 1 April 2014 (CDT) I might move the "How the CRISPR/Cas system works" section to part of the introduction. Perhaps this would follow a first paragraph, which ends with hints of using CRISPR/Cas for genome engineering... and then describe the natural-occurring system and its elements... This is one idea, not sure if it is the best idea.
- --Dennis Mishler (talk) 08:38, 1 April 2014 (CDT)Applications of CRISPR/Cas should be more fleshed out. A single sentence describing some applications is okay, but we should also have more information regarding one or two experiments... and what about in mammalian cells? Or Embryos? Could you take 20 fertilized eggs, perform CRISPR/Cas engineering and get a designer baby? This last question is more speculative, but if you can easily re-engineer mammalian cells...
- --Gabriel Suarez (talk) 21:35, 1 April 2014 (CDT)
Thank you Dennis! Check out intro modifications, mostly as you suggested.
--Chen-Hsun Tsai (talk) 02:14, 4 April 2014 (CDT) The structure of this wiki is well organized and make the story easy to understand. I like you have the advantage and disadvantage compared, and the figures also help readers to understand the complicate mechanisms. I only have few minor things; for example, I think some statements need a reference, such as "Advantage: Works on all cell types", or some of them could be more specific, such as "Disadvantage: Efficiency: it can be improved". Another thing is you said CRISPR could still have many non-specific editing, so are there any interesting studies focusing on solving this?
--Liz (talk) 07:26, 4 April 2014 (CDT)Well done. I think the intro and description of how the system works in nature are both very clear. For the CRISPR/Cas gene editing I feel that you start speeding up in a way (if possible in an article?) and maybe skipping over some things that - if defined/explained/rearranged could make this section much more understandable. Ie invert the Type II best sequence: Because all that is needed in Type II is .... it is the best. What is Cas9 compared to other Cas? Anything special there? The three steps at the end seem like the interesting/ meaty part but it also read kinda crammed on the end. Also I really like the figure under Applications. A short description of what we are looking at - even just what the parts we are looking at (ie what is recognition sequence, what is nuclease etc ...) for clarity, and maybe elaboration on how some of the more complex editing takes place- will be nice and helpful. Overall good job!
