Difference between revisions of "Talk:CH391L/S14/Directed protein evolution"

Revision as of 06:09, 28 February 2014

--drewtack (talk) 20:18, 16 February 2014 (CST)I know you haven't given your presentation yet, but, looking through this I'm surprised you didn't mention Frances Arnold at all. There is a citation at the end from her group, but I can't actually find where it is cited in the wiki. You might consider some mention of her lab in the wiki, and you might want to look into source #9.

--Ella Watkins (talk) 20:56, 27 February 2014 (CST)I noticed that there was no detail about the amplification step, was that left blank on purpose? And, after the genes have been selected/screened and amplified, what do they do with them? Are they inserted into plasmids etc...?

--Gabriel Suarez (talk) 23:59, 27 February 2014 (CST) I think I mentioned this before, but the Screening and Selection introduction paragraph is a bit confusing, from stating the need for "high-throughput" without really clearing out why (although I know you mentioned earlier that it is very difficult to get 'advantageous' mutations) to linking protein to DNA. I would reconsider re-writing it. Small grammar thing on first paragraph..."This methods is", I guess you meant "These methods are".

--Dennis Mishler (talk) 08:24, 11 February 2014 (CST) Gabo's Critique

Overall Format and structure: Nice layout, practical and overall easy to follow. Just a mini-detail: take off the ‘s’ from “This methods” in third sentence. Also, the PACE method kind of interrupts the flow of the report. Maybe it should have another title like “Novel methods for directed evolution” and would then go on to describe this method.

Introduction and background material:

Although short, I found introduction and background to be good and precise enough.

Methods and main body/concepts:

The description of error-prone PCR is quite good, but should also mention the need to avoid using polymerases with proofreading capabilities. The section on “Screening and selection” is a bit confusing and kind of jumps steps giving an incomplete view. Why would DNA be easier to isolate (I know it’s kind of obvious but should say “compared to proteins”) and why is it an ideal tag for proteins? (I guess it just needs rephrasing) Why the need for high-throughput?

Consider what the Lim 2002 paper said: “High-throughput assays are essential for determining the activity of the large % of all proteins whose functions are not known.” or specify that what has really been difficult is the development of high-throughput assays to test millions of proteins at once.

Relation to iGEM and future directions:

None found? I didn’t check around much but I guess this one is somehow related: http://sb6.biobricks.org/poster/engineering-transcription-with-a-novel-platform-for-directed-evolution/

Figures, Figure legends, and citations:

There are very good images around for Screening and selection methods. It would be nice to include one of these showing the basic methods such as physical linkage, phage display, ribosome display and such.