CH391L/S14/Ashley's First assignment
Ashley Kessel's first assignment: Cloned wiki (Feedback in discussion)
The ability to introduce exogenous DNA into an organism to alter its genetic program is one of the most crucial tools in modern biology. Early work showed that certain bacteria could acquire the traits of a related strain through the addition of heat-killed cells. Although it was not well understood at the time, the transfer of gene-encoding DNA from one strain to another facilitated this. This concept was turned into a useful tool upon the advent of bacterial plasmid transformations in the early 1970's, which allowed genes of interest to be easily inserted into E. coli. Over the years, methods have been developed to introduce exogenous genes into a wide range of useful organisms, including bacteria, yeasts, plants, and animal tissues. These methods vary enormously in efficiency however, necessitating a way to identify and isolate cells which contain the DNA of interest. This can be accomplished either by screening for successfully modified cells, or through selection.
Screening vs. Selection
The process of screening requires checking every cell (or colony in the case of bacteria) for the gene(s) of interest. This is often done with the aid of a screenable marker, like a fluorescent protein expressing gene, that allows rapid identification of transformed cells. Alternatively, exogenous genes in bacterial colonies can be detected by PCR or a variety of other methods without the use of a marker. Screening is often time-consuming and expensive, particularly when the fraction of modified cells is low. A much more efficient strategy, selection, involves growing cells in conditions which only allow for survival of those with the desired genes. Selectable marker genes can be combined with any other genes of interest and used to select for their insertion without additional screening steps. In bacteria, the most commonly used selectable markers provide resistance to antibiotics, allowing for positive selection of plasmids following transformation.
Antibiotic Resistance Markers: ampR
Perhaps the most commonly used selectable marker in bacteria is the ampR gene, which provides resistance to certain beta-lactam antibiotics such as ampicillin (amp) and its more stable relative carbenicillin (carb). Beta-lactam antibiotics are penicillin derivatives that inhibit synthesis of bacterial peptidoglycan cell walls, arresting cell division and ultimately leading to cell death. Although beta-lactams are primarily functional against gram-positive bacteria due to their larger cell walls, some examples, such as amp and carb, are capable of killing gram-negative E. coli. All antibiotics in the family share a central four atom ring structure known as the beta-lactam ring, which serves as a cleavage target for enzymes known as beta-lactamases. Due to the shared structural features of beta-lactam antibiotics, these enzymes often have promiscuous activities that target multiple drugs. Upon cleavage of the lactam ring, antibiotics such as amp lose their toxicity, allowing cell growth and division to resume.
The common ampR gene, as used in the E. coli pBR322 plasmid, was naturally derived from Salmonella bacteria through transposition. Because it efficiently cleaves amp and carb, it's one of the most useful markers in E. coli. Ampicillin does not kill the bacteria quickly, so cells transformed with the ampR marker can be immediately plated on selective media, before they've produced a significant amount of beta-lactamase. One downside to the use of amp is that it's rapid removal from selective media by beta-lactamase can allow for growth of cells that lack resistance. This is often witnessed in the form of satellite colonies on amp plates. The use of carbenicillin for selection reduces the problem, as carb is less rapidly degraded by the ampR beta-lactamase, but carb is significantly more expensive than amp. 
Marker genes that provide resistance to kanamycin (kan) and chloramphenicol (cap) are also popular for use in E. coli, as kan and cap are more stable than ampicillin. Bacterial cells transformed with kan and cap resistance markers must be allowed to recover before plating however. Additionally, multiple antibiotics may also be necessary to simultaneously select for markers on separate plasmids. For more information about how they work, see the links below:
Kanamycin on Wikipedia
Chloramphenicol on Wikipedia
Antibiotic/Non-Antibiotic Dual Selection: tetA(C)
Tetracycline Positive Selection
The tetA(C) gene is primarily used for positive selection in bacteria, similar to ampR. tetA(C) encodes a membrane-bound transporter that rapidly pumps the antibiotic tetracycline out of bacterial cells. This process is energy dependent, as the protein uses the influx of H+ ions from the surrounding environment to drive the process. Tetracycline is a broad-spectrum, polyketide antibiotic derived from Streptomyces that inhibits bacterial translation. The antibiotic binds the 30s subunit of bacterial ribosomes, blocking entry of aminoacyl-tRNAs to the A site of the ribosome.
[[ The structure of tetracycline. Tetracycline on Wikipedia]]
Because tetracycline resistance is gained through expression of a transporter and not a modifying enzyme, selective media maintains its antibiotic levels during growth of resistant cells. High levels of tetA expression can have a detrimental effect on the cells however, due the energy cost of pumping tetracycline and increased vulnerability to certain extracellular conditions. This often results in fewer viable cells when using tetracycline selection compared to selection with antibiotics like ampicillin and kanamycin.
Nickel Salt Negative Selection
Expression of tetA in bacteria has the side effect of making cells more vulnerable to poisoning by either lipophilic chelating agents or metal salts. Although tetA expressing Salmonella strains were shown to be extremely sensitive to chelating agents such as fusaric or quinaldic acids, these compounds are only marginally effective on E. coli. Based on earlier observations that metals like cadmium inhibited growth of tetracycline-resistant bacteria, a technique was developed that uses nickel salts to select against tetA expressing E. coli with much greater efficiency. Cheap, non-toxic Nickel Chloride is the most commonly used selection agent. Although originally used to select for cells that had lost a tetA marker, this method has proven useful in dual selection schemes for evolving regulators of gene expression. [4, 5]
Engineering Riboswitches using tetA Dual Selection
The evolution of functional Riboswitches requires selection of a library of mutants in both an ON state and an OFF state. This removes variants that either activate or repress gene expression regardless of the small molecule used to regulate them. Previous attempts at dual selection required the use of both positive and negative selection markers. This necessitated intermediate steps to purify plasmids from the pool and re-transform them along with either the positive or negative marker. Aside from being labor intensive, this method also increased the rate of false positives in the pool [6, 7].
The fact that a single tetA gene can be used for both positive and negative selection greatly simplifies the process of selecting for riboswitches, as a culture of cells expressing a library of riboswitches can be alternately swapped between media that selects for or against expression of the tetracycline transporter. This strategy was used by the Yokobayashi Lab at UC Davis to select for mutants of the E. coli TPP riboswitch (from thiM) that activated downstream gene expression in the presence of thiamine instead of repressing it. A library of TPP riboswitch variants was cloned upstream of a tetA marker and transformed into E. coli. The first round of selection involved plating transformants on media with thiamine and tetracycline to select for mutants that were turned ON. The survivors were then transferred to media with nickel chloride and no thiamine to remove any variants that did not turn off in the absence of thiamine. A similar approach can be carried out in liquid culture, as shown below..
Using a tetA Fusion Protein for Monitoring Selection
More recent work by the Yokobayashi Lab involved combining the tetA marker with GFP to allow screening of thiM riboswitch function following each round of dual selection. The tetA transporter remains functional when GFP is fused to its c-terminal end, facilitating selection and screening steps with the same gene product. To measure function of different riboswitch variants in the selection pool, samples were plated on LB and individual colonies were picked and used to seed culture in non-selective media. GFP fluorescence of each culture was measured to determine the amount of gene expression generated by that particular riboswitch, and functional variants were sequenced.
Auxotrophic Marker Selection: GFAT
An alternative method of positive selection involves the use of auxotrophs, or cells that lack the ability to synthesize a compound necessary for their own growth. In bacteria and yeast, useful auxotrophic strains are easily generated by knocking out a single gene involved in a chemical synthesis pathway. The resulting strains can be grown in media containing the essential product but require complementation with an exogenous copy of the missing gene (the auxotrophic marker) to remain viable in media lacking it. Auxotrophic markers are extremely useful in yeast, where the range of useful antibiotic markers is limited, but they are also used in bacterial applications where introduction of antibiotic-resistance genes could be problematic. Recently, a single gene involved in glucosamine synthesis was shown to be a useful auxotrophic marker in E. coli and fission yeast (S. pombe).
Glutamine:fructose-6-phosphate aminotransferase (GFAT) is an enzyme required for the biosynthesis of hexosamines in both bacteria and eukaryotes. GFAT catalyzes the rate-limiting first step in the pathway, converting fructose-6-phosphate into glucosamine-6-phosphate. In E. coli, GFAT is expressed from the glmS gene, which is necessary for growth in minimal media. Recent work has shown that E. coli lacking glmS are capable of growing at near wild type levels when supplemented with glucosamine however, and complementation with a plasmid-encoded glmS gene restores hexosamine synthesis and growth.
Selection of glmS expressing cells is complicated by the fact that rich media such as LB contains sufficient glucosamine to support limited growth of glmS knockout cells. Growth tests using M9 minimal media with individual LB components added to it showed that yeast extract is the source of glucosamine that allows growth of the knockout strain. Thus, selections with GFAT markers are limited to minimal media, but the addition of tryptone increases growth rates while still allowing selection. Under these conditions, selection for glmS was shown to be an effective replacement for ampR selection.
Although the currently available set of selectable markers has helped us to overcome many of the challenges involved in synthetic biology, the continued discovery of new markers may greatly simplify the process of designing organisms with useful functions. The variety of useful markers available for engineering of higher eukaryotes, including animals and human tissue culture lines pales in comparison to those available for use in bacteria or even yeast. Thus, the discovery of new selection schemes may allow experiments that are not currently feasible.
Another area of ongoing progress is the discovery of non-antibiotic markers to potentially replace commonly used genes like ampR in bacterial engineering. Although antibiotic resistance is an increasingly common trait in wild bacteria, the possibility of engineered organisms transferring their resistance to otherwise susceptible bacteria is a frequent cause for alarm. If engineered bacteria are to prove useful for tasks like bio-remediation, we will have to engineer them without the benefit of antibiotic selection or markers that require growth in minimal media.
The 2011 Peking_R iGEM team from Peking University used tetA dual selection to engineer a ribozyme-based "genetic rheostat." Their approach involved fusing the TPP aptamer domain from the thiM riboswitch to a section of the hammerhead ribozyme. Portions of the construct were randomized and selections were carried out in a similar fashion to those performed by the Yokobayashi Lab. This approach did increase the ratio of ON/OFF ribozymes in their pool, and similar selections using an adenine aptamer domain also demonstrated the potential of the approach.
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CaCl2 bacterial transformations
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Background on the ampR gene from pBR322
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The tetA(C) gene from pBR322
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Nickel selection with tetA
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Reengineering the TPP riboswitch using tetA dual selection
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Riboswitch selection/screening using a tetA-GFP fusion marker
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GFAT as a selectable marker