Difference between revisions of "Analog Genetic Circuits (Microbes as Machines)"

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==== [[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]] ====
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[[File:repressilator_plasmids.png|400px|thumb|right|Repressilator <cite>Gibson2010</cite>. Image from [http://en.wikipedia.org/wiki/Repressilator Wikipedia article].]]  
  
[[File:repressilator_plasmids.png|300px|thumb|right|Repressilator <cite>Gibson2010</cite>. Image from [http://en.wikipedia.org/wiki/Repressilator Wikipedia article].]]  
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==== [[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]] ====
  
 
<blockquote>
 
<blockquote>
<font size="4">The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process <cite>Elowitz2000</cite>.</font>
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<font size="4">Such 'rational network design' may lead both to the engineering of new cellular behaviours and to an improved understanding of naturally occurring networks <cite>Elowitz2000</cite>.</font>
 
</blockquote>
 
</blockquote>
  
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#Elowitz2000 pmid=10659856
 
#Elowitz2000 pmid=10659856
 
// Describes the artificial "repressilator" circuit.
 
// Describes the artificial "repressilator" circuit.
#Anderson2014 Toggle Switch and Repressilator ([https://www.youtube.com/watch?v=0ksvU6Bckbk YouTube])
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#Gardner2000 pmid=10659857
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// Describes an artificial "toggle switch" circuit.
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#Anderson2014 Overview of the Toggle Switch and Repressilator ([https://www.youtube.com/watch?v=0ksvU6Bckbk YouTube])
 
</biblio>
 
</biblio>
  
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<biblio>
 
<biblio>
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#Friedland2009 pmid=19478183
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// Bacteria that can count to three.
 
#Prindle2012 pmid=22178928
 
#Prindle2012 pmid=22178928
 
// Synchronization and modulation of oscillations across multiple cell populations.
 
// Synchronization and modulation of oscillations across multiple cell populations.

Revision as of 06:08, 16 February 2015

Repressilator [1]. Image from Wikipedia article.

Contents

Download Pre-Discussion Questions (PDF)

Such 'rational network design' may lead both to the engineering of new cellular behaviours and to an improved understanding of naturally occurring networks [2].

Required Reading/Viewing

Error fetching PMID 10659856:
Error fetching PMID 10659857:
  1. Error fetching PMID 10659856: [Elowitz2000]
    Describes the artificial "repressilator" circuit.
  2. Error fetching PMID 10659857: [Gardner2000]
    Describes an artificial "toggle switch" circuit.
  3. Overview of the Toggle Switch and Repressilator (YouTube) [Anderson2014]
All Medline abstracts: PubMed | HubMed

Assigned Papers

Error fetching PMID 19478183:
Error fetching PMID 22178928:
  1. Error fetching PMID 19478183: [Friedland2009]
    Bacteria that can count to three.
  2. Error fetching PMID 22178928: [Prindle2012]
    Synchronization and modulation of oscillations across multiple cell populations.
All Medline abstracts: PubMed | HubMed

Additional Resources

  1. Repressilator in TinkerCell(YouTube) [Chandran2011]
    Movie shows how to create and model the repressilator in TinkerCell