Difference between revisions of "DNA Assembly Methods(Microbes as Machines)"

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(Created page with "Prototypical 'datasheet' <cite>Canton2008</cite>. ==== Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Do...")
 
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[[File:Synthia_Genome.png|300px|thumb|right|Prototypical 'datasheet' <cite>Canton2008</cite>.]]  
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[[File:Synthia_Genome.png|300px|thumb|right|Construction of the synthetic genome of ''Mycoplasma laboratorium'' <cite> Jiang2013 </cite>.]]  
  
 
==== [[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]] ====
 
==== [[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]] ====
  
 
<blockquote>
 
<blockquote>
<font size="4">The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process.<cite>Gibson2010</cite>.</font>
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<font size="4">The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process <cite>Gibson2010</cite>.</font>
 
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<biblio>
 
<biblio>
#Gibson2009 pmid=19363495  
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#Gibson2009 pmid=19363495
 
// Describes isothermal DNA assembly (aka Gibson Cloning)
 
// Describes isothermal DNA assembly (aka Gibson Cloning)
 
</biblio>
 
</biblio>
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<biblio>
 
<biblio>
 
#Gibson2010 pmid=20488990
 
#Gibson2010 pmid=20488990
// Construction of synthetic M. mycoides genome
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// Construction of synthetic ''Mycoplasma laboratorium'' genome
 
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#Jiang2013 pmid=23360965
// Describes CRISPR-based genome editing
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// Describes CRISPR-based genome editing of ''Escherichia coli''
 
</biblio>
 
</biblio>

Revision as of 19:39, 8 February 2015

Construction of the synthetic genome of Mycoplasma laboratorium [1].

Download Pre-Discussion Questions (PDF)

The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [2].

Required Reading

Error fetching PMID 19363495:
  1. Error fetching PMID 19363495: [Gibson2009]
    Describes isothermal DNA assembly (aka Gibson Cloning)

Assigned Papers

Error fetching PMID 20488990:
Error fetching PMID 23360965:
  1. Error fetching PMID 23360965: [Jiang2013]
    Describes CRISPR-based genome editing of Escherichia coli
  2. Error fetching PMID 20488990: [Gibson2010]
    Construction of synthetic Mycoplasma laboratorium genome
All Medline abstracts: PubMed | HubMed