DNA Assembly Methods(Microbes as Machines)

DNA Assembly Methods(Microbes as Machines)

Construction of the synthetic genome of Mycoplasma laboratorium [1].

Download Pre-Discussion Questions (PDF)

The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [2].

Required Reading

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3. Describes isothermal DNA assembly (aka Gibson Cloning)

Assigned Papers

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1. Describes CRISPR-based genome editing of Escherichia coli

2. Construction of synthetic Mycoplasma laboratorium genome