DNA Assembly Methods(Microbes as Machines)

DNA Assembly Methods(Microbes as Machines)

Required Reading

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1. Describes isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website.

Construction of the synthetic genome of Mycoplasma laboratorium [2].

Assigned Papers

The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [2].

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2. Construction of synthetic Mycoplasma laboratorium genome

3. Describes CRISPR-based genome editing of Escherichia coli