DNA Assembly Methods(Microbes as Machines)

DNA Assembly Methods(Microbes as Machines)

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Construction of the synthetic genome of Mycoplasma laboratorium [1].

The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [1].

Required Reading/Viewing

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1. Construction of synthetic Mycoplasma laboratorium genome.

2. Venter press conference / TED Talk discussing this paper

Assigned Papers

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3. Pligonucleotide and gene synthesis methods

4. Isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website.

5. In vivo DNA assembly using homologous recombination in yeast.