Talk:CH391L/S14/Gibson Assembly
From SynBioCyc
- --Ella Watkins (talk) 18:53, 3 February 2014 (CST) I would really like to see some of the illustrations that you had to go along with your wiki. Your presentation was amazing and I really think that the illustrations helped me visualize the concept a lot better. Also in backgrounds: maybe elaborate a tiny bit on why they chose T5, the exonuclease that is unstable at higher temperatures. In your presentation you did a good job telling us it was chosen so that the temperature could be raised and the T5 would no longer function. In the wiki, it mentions that it is not thermostable, but doesn't really tell why.
- --Dennis Mishler (talk) 12:23, 5 February 2014 (CST) I completely agree with Ella on the figures/illustrations. You can probably just adapt the figures from your presentation. Visual representation will greatly increase the ability of other to read and understand your wiki page.
- --drewtack (talk) 13:40, 6 February 2014 (CST)It is quite well explained. Might I suggest mentions how it has been useful in synthetic genome assembly. You could use the mouse mitochondria paper, which you mentioned in your presentation, as well as the Mycoplasma mycoides paper to show examples where Gibson Assembly has been utilized in mainstream research. Additionally, you only have one listed source, which is not a big deal, but you also don't have many links to other cites in your wiki, which i think would be helpful. I'm all for those external links, wiki is like the thoughtweb of the internet.
