DNA Assembly Methods(Microbes as Machines)

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Download Pre-Discussion Questions (PDF)

Construction of the synthetic genome of Mycoplasma laboratorium [1].
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [1].

Required Reading/Viewing

Error fetching PMID 20488990:
  1. Error fetching PMID 20488990: [Gibson2010]
    Construction of synthetic Mycoplasma laboratorium genome.
  2. Venter press conference / TED Talk discussing this paper [Venter2010]

Assigned Papers

Error fetching PMID 21601682:
Error fetching PMID 19363495:
Error fetching PMID 24028550:
Error fetching PMID 19745056:
  1. Error fetching PMID 21601682: [Hughes2011]
    Oligonucleotide and gene synthesis methods
  2. Error fetching PMID 19363495: [Gibson2009a]
    Isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website.
  3. Error fetching PMID 24028550: [Kuijpers2013]
    1/2 In vivo DNA assembly using homologous recombination in yeast
  4. Error fetching PMID 19745056: [Gibson2009b]
    2/2 In vivo assembly of single-stranded oligonucleotides in yeast
All Medline abstracts: PubMed | HubMed

Additional Information