Difference between revisions of "DNA Assembly Methods(Microbes as Machines)"

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[[File:Synthia_Genome.png|300px|thumb|right|Construction of the synthetic genome of ''Mycoplasma laboratorium'' <cite> Jiang2013 </cite>.]]
 
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==== [[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]] ====
 
==== [[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]] ====
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[[File:Synthia_Genome.png|300px|thumb|right|Construction of the synthetic genome of ''Mycoplasma laboratorium'' <cite>Gibson2010</cite>.]]
  
 
<blockquote>
 
<blockquote>
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= Required Reading =
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= Required Reading/Viewing =
  
 
<biblio>
 
<biblio>
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#Gibson2009 pmid=19363495
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#Gibson2010 pmid=20488990
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// Describes isothermal DNA assembly (aka Gibson Cloning)
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// Construction of synthetic ''Mycoplasma laboratorium'' genome.
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#Venter2010 [http://www.ted.com/talks/craig_venter_unveils_synthetic_life?language=en Venter press conference / TED Talk discussing this paper]
 
</biblio>
 
</biblio>
  
 
= Assigned Papers =
 
= Assigned Papers =
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<biblio>
 
<biblio>
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#Gibson2010 pmid=20488990
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#Hughes2011 pmid=21601682
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// Construction of synthetic ''Mycoplasma laboratorium'' genome
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// Oligonucleotide and gene synthesis methods
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#Jiang2013 pmid=23360965
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#Gibson2009a pmid=19363495
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// Describes CRISPR-based genome editing of ''Escherichia coli''
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// Isothermal DNA assembly (aka Gibson Cloning). Additional information on the [https://www.addgene.org/plasmid-protocols/gibson-assembly/ AddGene website].
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#Kuijpers2013 pmid=24028550
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// 1/2 In vivo DNA assembly using homologous recombination in yeast
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#Gibson2009b pmid=19745056
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// 2/2 In vivo assembly of single-stranded oligonucleotides in yeast
 
</biblio>
 
</biblio>
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= Additional Information =
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[[File:Synthia_Genome_Assembly_Flight.jpeg|thumbnail|500px|left|Image from [https://cmcagustinos.wordpress.com/2011/02/07/genoma-sintetico-y-celula-artificial/ Genoma sintético y célula artificial]]]
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[[File:Venter_Defragmented_Minimal_Genome_Slide.png|thumbnail|500px|left|Image from [https://twitter.com/hashtag/sbbsf14 Image from #SBBSF14]]]

Latest revision as of 18:46, 13 February 2015

Contents

Download Pre-Discussion Questions (PDF)

Construction of the synthetic genome of Mycoplasma laboratorium [1].
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [1].

Required Reading/Viewing

Error fetching PMID 20488990:
  1. Error fetching PMID 20488990: [Gibson2010]
    Construction of synthetic Mycoplasma laboratorium genome.
  2. Venter press conference / TED Talk discussing this paper [Venter2010]

Assigned Papers

Error fetching PMID 21601682:
Error fetching PMID 19363495:
Error fetching PMID 24028550:
Error fetching PMID 19745056:
  1. Error fetching PMID 21601682: [Hughes2011]
    Oligonucleotide and gene synthesis methods
  2. Error fetching PMID 19363495: [Gibson2009a]
    Isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website.
  3. Error fetching PMID 24028550: [Kuijpers2013]
    1/2 In vivo DNA assembly using homologous recombination in yeast
  4. Error fetching PMID 19745056: [Gibson2009b]
    2/2 In vivo assembly of single-stranded oligonucleotides in yeast
All Medline abstracts: PubMed | HubMed

Additional Information

Image from Genoma sintético y célula artificial
Image from Image from #SBBSF14
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