DNA Assembly Methods(Microbes as Machines)
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Download Pre-Discussion Questions (PDF)
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process [1].
Required Reading/Viewing
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Construction of synthetic Mycoplasma laboratorium genome. - Venter press conference / TED Talk discussing this paper
Assigned Papers
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Oligonucleotide and gene synthesis methods - Error fetching PMID 19363495:
Isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website. - Error fetching PMID 24028550:
1/2 In vivo DNA assembly using homologous recombination in yeast - Error fetching PMID 19745056:
2/2 In vivo assembly of single-stranded oligonucleotides in yeast