DNA Assembly Methods(Microbes as Machines)
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Download Pre-Discussion Questions (PDF)
The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process.[2].
Required Reading
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- Error fetching PMID 19363495:
Describes isothermal DNA assembly (aka Gibson Cloning)
Assigned Papers
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- Error fetching PMID 20488990:
Construction of synthetic M. mycoides genome // Describes CRISPR-based genome editing