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• CH391L/S14/Biocontainment
  ...te laboratory synthesized genetic information fall into two broad classes: 1) genetically encoded dependence systems and 2) semantic firewalls. ...idt2012</cite>. Considering there may be ten times that number of cells in 1 mL of culture, the fundamental problem of mutations to these strategies is
  9 KB (1,376 words) - 19:23, 3 March 2014
• CH391L/S14/NucleicAcidsRiboswitches
  '''Read the Abstract, introduction, and figure 1 of:''' '''Read the abstract, introduction, and figure 1 of:'''
  551 B (76 words) - 18:29, 26 February 2014
• CH391L/S14/StochasticGeneExpression
  '''kR*P[m] = γR*(m+1)*P[m+1]''' ...ing an additional copy, which will equal to the rate of degradation from m+1 transcript if equilibrium is possible. The equation can only be possible wh
  9 KB (1,329 words) - 23:18, 6 March 2014
• CH391L/S14/Spinach RNA
  ...//scholar.google.com/scholar?rlz=1C1CHFX_enUS569US569&espv=210&es_sm=93&um=1&ie=UTF-8&lr=&cites=15840443076454798134 120 articles]. ...nded by forming RNA granules colocalized with T-cell intracellular antigen-1-related protein, as was expected of the stress conditions.
  7 KB (1,105 words) - 15:46, 17 March 2014
• CH391L/S14/ApplicationsofOptogenetics
  *Step 1: '''Identify potential light sensitive proteins:''' Light sensitive protein 1. Opsin gene is combined with a promotor to ensure the gene is active only i
  14 KB (2,005 words) - 18:55, 24 March 2014
• CH391L/S14/QuorumSensing
  ...classes of autoinducers, Autoinducer 1 (AI-1) and Autoinducer 2 (AI-2). AI-1's are used for intraspecies communication and differ between gram negative '''AI-1'''
  12 KB (1,789 words) - 15:57, 24 March 2014
• CH391L/S14/MotilityandTaxis
  [[File:Goe_chemo1.png|thumb|right|300px|Figure 1: Chemotaxis of E. coli. Left: when no attractant is present E. coli switche ...ls to travel to areas more favorable for their survival and growth (Figure 1).
  17 KB (2,489 words) - 19:41, 24 March 2014
• CH391L/S14/Cellcountingmemory
  ...pressor 1 inhibits transcription from Promoter 1 and is induced by Inducer 1. Repressor 2 inhibits transcription from Promoter 2 and is induced by Induc ...both counters, the first coding region has a constitutive promoter, PLtet0-1, and all coding regions have a cis-acting repressor (cr) that forms a stem-
  15 KB (2,415 words) - 15:11, 31 March 2014
• CH391L/S14/Oscillation
  ...tic networks designed to execute a particular function. As shown in figure 1, the Repressilator has three the repressor genes (tetR-lite, lac-lite, and
  11 KB (1,746 words) - 17:21, 31 March 2014
• CH391L/S14/CRISPR
  ...014.JPG|thumb|325 px|left|'''The three stages of the CRISPR/Cas system''' (1) adaptation, (2) crRNA biogenesis and (3) invader silencing (interference). ...hrough Jinek 2012 crRNA and tracrRNA chimera.JPG|thumb|200 px|right|'''Fig.1''' Cas9 using a single engineered RNA molecule combining tracrRNA and crRNA
  16 KB (2,312 words) - 22:02, 8 April 2014
• CH391L/S14/MAGE
  ...AGE can be used to target specific sequences with well-defined oligos, the 1-deoxy-D-xylulose-5-phosphate or DXP synthesis pathway responsible for lycop ... ''E. coli'' has three stop codons and two release factors. Release factor 1 (RF1) recognizes UAA and UAG, while RF2 recognizes UAA and UGA. The hypothe
  12 KB (1,843 words) - 14:29, 31 March 2014
• CH391L/S14/SmallRNAs
  ...onal control. Translational control is imparted by sRNAs in various ways: (1) A sRNA base-pairs to its target mRNA sequestering the Ribosome-Binding Sit ...A expression this class of sRNAs uses a diversity of mechanisms. They can (1) base-pair to their target mRNAs to enhance or attenuate transcription (Fig
  18 KB (2,689 words) - 18:27, 14 April 2014
• CH391L/S14/NitrogenFixationRefactoring
  ...rs are frequently subject to complex and highly redundant host regulation [1]. Furthermore, there may be no laboratory conditions known that allow for e
  10 KB (1,493 words) - 19:43, 17 April 2014
• CH391L/S14/Bioremediation&caffeinatedcoli
  ...t al. engineered ''E. coli'' and made it capable of biodegrading caffeine (1,3,7-trimethylxanthine). This strain can transform caffeine into xanthine, w
  9 KB (1,299 words) - 16:04, 17 April 2014
• CH391L/S14/Artemisinin
  ...ication. [[Image:EG042114Artemisinin.png | thumb | right | 250 px | Figure 1:Structure of artemisinin ]] ...crease overall flux into the artemisinin synthesis pathway, enzymes of the 1-deoxy-D-xylulose 5-phosphate (DXP) pathway, which produces FPP in ''E. coli
  10 KB (1,397 words) - 15:17, 21 April 2014
• Spring 2015 Microbes as Machines
  | 1 || Jan 26 || [[What is Synthetic Biology? (Microbes as Machines)|What is Sy
  2 KB (214 words) - 13:21, 7 November 2016
• Microbe Hackers
  ...wing the cells to grow. Figure from Quandt, et al. 2013; "Caffeinated Coli 1.0".]] The original, "Version 1.0" was pioneered by the UT Austin iGEM team with Professor Barrick. This s
  30 KB (4,738 words) - 14:20, 14 October 2022
• DNA Assembly Methods(Microbes as Machines)
  // 1/2 In vivo DNA assembly using homologous recombination in yeast
  2 KB (200 words) - 18:46, 13 February 2015
• Fall 2016 Biotechnology and Society
  | 1 || Sept 12 || [[Dual-Use Research (Biotechnology and Society 2016)|Dual-Use
  2 KB (258 words) - 20:33, 28 November 2016
• Recombinant DNA Technology (Biotechnology and Society 2016)
  ...ant DNA molecule. You can skim the paper. The main result is shown in Fig. 1.
  2 KB (358 words) - 16:24, 29 August 2016