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  • Fall 2013 Biotechnology and Society
    | 1 || Sept 9 || [[Recombinant DNA Technology (Biotechnology and Society)|Recom
    2 KB (187 words) - 22:20, 14 December 2013
  • Recombinant DNA Technology (Biotechnology and Society)
    ...ant DNA molecule. You can skim the paper. The main result is shown in Fig. 1.
    2 KB (358 words) - 02:24, 4 September 2013
  • Do-It-Yourself (DIY) Biology (Biotechnology and Society)
    ...public participation in the life sciences today. ''J. Sci. Comm.'' '''9''':1-8. [http://jcom.sissa.it/archive/09/01/Jcom0901(2010)C01/Jcom0901(2010)C03
    2 KB (333 words) - 01:47, 22 October 2013
  • In vitro Meat (Biotechnology and Society)
    #PETA PETA Offers $1 Million Reward to First Person to Make 'Test Tube' Meat
    2 KB (286 words) - 18:07, 7 October 2013
  • Chemical Synthesis of Life (Biotechnology and Society)
    ... [http://www.ibiology.org/ibioseminars/evolution-ecology/jack-szostak-part-1.html Watch on iBiology.org]
    2 KB (222 words) - 21:19, 13 November 2016
  • Spring 2014 Synthetic Biology
    === Week 1: Introduction ===
    6 KB (814 words) - 19:32, 7 May 2014
  • CH391L/S14/WikiEditing
    // Synthesis of an entire 1.08 Mbp ''Mycoplasma mycoides'' genome from scratch and successful transplan // Synthesis of an entire 1.08 Mbp ''Mycoplasma mycoides'' genome from scratch and successful transplan
    12 KB (1,918 words) - 09:03, 17 January 2014
  • CH391L/S14/First Assignment
    ...e code. Then, go to your First assignment link, which you created in step 1. Paste the entire source code into the editor.
    3 KB (455 words) - 19:35, 24 January 2014
  • CH391L/S14/FinalProjectRubric
    * '''Abstract''' (1 paragraph, 250 words max) * '''Motivation''' (1-3 paragraphs)
    4 KB (596 words) - 17:45, 10 January 2014
  • CH391L/S14/Dennis's First assignment
    ...leading to stronger evidence for extremely warm global conditions prior to 1 Gya<cite>Gaucher2</cite>. Archaic proteins also allow for greater study of
    11 KB (1,628 words) - 03:12, 13 January 2014
  • CH391L/S14/In class First assignment
    ... by the P1 and R1 genes.<cite>Gardner2000</cite> In pIKE, P1 is the PLtetO-1 promoter and R1 is ''tetR''. This toggle switch is flipped by IPTG or aTc p
    9 KB (1,448 words) - 19:00, 20 January 2014
  • CH391L/S14/BioBricks
    ...trand of the target DNA at different spots creating 3'- or 5'-overhangs of 1 to 4 nucleotides (so-called sticky ends). <cite> Sambrook1989</cite> There {| class="wikitable sortable" border="1" cellpadding="4" cellspacing="0" style="border:1px solid #aaa; border-colla
    15 KB (2,291 words) - 16:06, 31 January 2014
  • CH391L/S14/Jordan's First Assignment
    .... For a several kilobase gene fragment, oligo cost alone can still exceed $1,000. To over come this hurdle, multiplex parallel gene synthesis technologi ... chemistry. Phosphoramidite oligo synthesis currently has an error rate of 1 in ~160. This results in a high enough error rate in assembled genes that s
    22 KB (3,195 words) - 03:19, 17 January 2014
  • CH391L/S14/Gabo's First Assignment
    ...difficile'' infection was to assessing patient to one of three treatments: 1) included a 4 day [http://en.wikipedia.org/wiki/Vancomycin vancomycin] trea ... the balance between these two markers, if too much NO is sensed by Device 1 it would prevent inflammation. The opposite would also be true if the secon
    9 KB (1,408 words) - 09:09, 17 January 2014
  • CH391L/S14/Drew's First assignment
    ... by the P1 and R1 genes.<cite>Gardner2000</cite> In pIKE, P1 is the PLtetO-1 promoter and R1 is ''tetR''. This toggle switch is flipped by IPTG or aTc p
    9 KB (1,442 words) - 19:05, 20 January 2014
  • CH391L/S14/Chen-Hsun's First assignment
    ...re peak of the EPR spectrum. (C) Relative fluorescence of reduced aptamers 1–3, plotted as a function of cocaine concentration in an aqueous solution ...times however with each successive test, the accuracy of the test drops by 1%. Scott Test works in a similar way. The benefit of detecting cocaine in th
    11 KB (1,707 words) - 21:44, 23 January 2014
  • CH391L/S14/CAD systems
    '''step 1''': User draws a biological system <br> '''step 2''': User performs some analysis and repeats step 1 if didn't obtain what expected
    20 KB (2,869 words) - 19:41, 3 February 2014
  • CH391L/S14/Genome Synthesis
    ...evisiae'' was also used to assemble the final genome, with a total size of 1.08 Mb. ...was removed at all 321 natural sites in ''E coli'', and the release factor 1 (''prf1'')gene was removed. These modifications served two purposes; first
    14 KB (2,036 words) - 17:31, 10 February 2014
  • CH391L/S14/Gibson Assembly
    0.8 µl of 0.2 U µl–1 or 1.0 U µl–1 T5 exonuclease<br> 4 µl of 40 U µl–1 Taq DNA ligase<br>
    7 KB (1,163 words) - 21:18, 6 February 2014
  • CH391L/S14/Ancestral sequence
    ...logy, and improved techniques allow reconstruction of genes from more than 1 billion years ago ...leading to stronger evidence for extremely warm global conditions prior to 1 Gya<cite>Gaucher2</cite>. Archaic proteins also allow for greater study of
    17 KB (2,472 words) - 00:49, 12 February 2014
  • CH391L/S14/Biocontainment
    ...te laboratory synthesized genetic information fall into two broad classes: 1) genetically encoded dependence systems and 2) semantic firewalls. ...idt2012</cite>. Considering there may be ten times that number of cells in 1 mL of culture, the fundamental problem of mutations to these strategies is
    9 KB (1,376 words) - 19:23, 3 March 2014
  • CH391L/S14/NucleicAcidsRiboswitches
    '''Read the Abstract, introduction, and figure 1 of:''' '''Read the abstract, introduction, and figure 1 of:'''
    551 B (76 words) - 18:29, 26 February 2014
  • CH391L/S14/StochasticGeneExpression
    '''kR*P[m] = γR*(m+1)*P[m+1]''' ...ing an additional copy, which will equal to the rate of degradation from m+1 transcript if equilibrium is possible. The equation can only be possible wh
    9 KB (1,329 words) - 23:18, 6 March 2014
  • CH391L/S14/Spinach RNA
    ...//scholar.google.com/scholar?rlz=1C1CHFX_enUS569US569&espv=210&es_sm=93&um=1&ie=UTF-8&lr=&cites=15840443076454798134 120 articles]. ...nded by forming RNA granules colocalized with T-cell intracellular antigen-1-related protein, as was expected of the stress conditions.
    7 KB (1,105 words) - 15:46, 17 March 2014
  • CH391L/S14/ApplicationsofOptogenetics
    *Step 1: '''Identify potential light sensitive proteins:''' Light sensitive protein 1. Opsin gene is combined with a promotor to ensure the gene is active only i
    14 KB (2,005 words) - 18:55, 24 March 2014
  • CH391L/S14/QuorumSensing
    ...classes of autoinducers, Autoinducer 1 (AI-1) and Autoinducer 2 (AI-2). AI-1's are used for intraspecies communication and differ between gram negative '''AI-1'''
    12 KB (1,789 words) - 15:57, 24 March 2014
  • CH391L/S14/MotilityandTaxis
    [[File:Goe_chemo1.png|thumb|right|300px|Figure 1: Chemotaxis of E. coli. Left: when no attractant is present E. coli switche ...ls to travel to areas more favorable for their survival and growth (Figure 1).
    17 KB (2,489 words) - 19:41, 24 March 2014
  • CH391L/S14/Cellcountingmemory
    ...pressor 1 inhibits transcription from Promoter 1 and is induced by Inducer 1. Repressor 2 inhibits transcription from Promoter 2 and is induced by Induc ...both counters, the first coding region has a constitutive promoter, PLtet0-1, and all coding regions have a cis-acting repressor (cr) that forms a stem-
    15 KB (2,415 words) - 15:11, 31 March 2014
  • CH391L/S14/Oscillation
    ...tic networks designed to execute a particular function. As shown in figure 1, the Repressilator has three the repressor genes (tetR-lite, lac-lite, and
    11 KB (1,746 words) - 17:21, 31 March 2014
  • CH391L/S14/CRISPR
    ...014.JPG|thumb|325 px|left|'''The three stages of the CRISPR/Cas system''' (1) adaptation, (2) crRNA biogenesis and (3) invader silencing (interference). ...hrough Jinek 2012 crRNA and tracrRNA chimera.JPG|thumb|200 px|right|'''Fig.1''' Cas9 using a single engineered RNA molecule combining tracrRNA and crRNA
    16 KB (2,312 words) - 22:02, 8 April 2014
  • CH391L/S14/MAGE
    ...AGE can be used to target specific sequences with well-defined oligos, the 1-deoxy-D-xylulose-5-phosphate or DXP synthesis pathway responsible for lycop ... ''E. coli'' has three stop codons and two release factors. Release factor 1 (RF1) recognizes UAA and UAG, while RF2 recognizes UAA and UGA. The hypothe
    12 KB (1,843 words) - 14:29, 31 March 2014
  • CH391L/S14/SmallRNAs
    ...onal control. Translational control is imparted by sRNAs in various ways: (1) A sRNA base-pairs to its target mRNA sequestering the Ribosome-Binding Sit ...A expression this class of sRNAs uses a diversity of mechanisms. They can (1) base-pair to their target mRNAs to enhance or attenuate transcription (Fig
    18 KB (2,689 words) - 18:27, 14 April 2014
  • CH391L/S14/NitrogenFixationRefactoring
    ...rs are frequently subject to complex and highly redundant host regulation [1]. Furthermore, there may be no laboratory conditions known that allow for e
    10 KB (1,493 words) - 19:43, 17 April 2014
  • CH391L/S14/Bioremediation&caffeinatedcoli
    ...t al. engineered ''E. coli'' and made it capable of biodegrading caffeine (1,3,7-trimethylxanthine). This strain can transform caffeine into xanthine, w
    9 KB (1,299 words) - 16:04, 17 April 2014
  • CH391L/S14/Artemisinin
    ...ication. [[Image:EG042114Artemisinin.png | thumb | right | 250 px | Figure 1:Structure of artemisinin ]] ...crease overall flux into the artemisinin synthesis pathway, enzymes of the 1-deoxy-D-xylulose 5-phosphate (DXP) pathway, which produces FPP in ''E. coli
    10 KB (1,397 words) - 15:17, 21 April 2014
  • Spring 2015 Microbes as Machines
    | 1 || Jan 26 || [[What is Synthetic Biology? (Microbes as Machines)|What is Sy
    2 KB (214 words) - 13:21, 7 November 2016
  • Microbe Hackers
    ...wing the cells to grow. Figure from Quandt, et al. 2013; "Caffeinated Coli 1.0".]] The original, "Version 1.0" was pioneered by the UT Austin iGEM team with Professor Barrick. This s
    30 KB (4,738 words) - 14:20, 14 October 2022
  • DNA Assembly Methods(Microbes as Machines)
    // 1/2 In vivo DNA assembly using homologous recombination in yeast
    2 KB (200 words) - 18:46, 13 February 2015
  • Fall 2016 Biotechnology and Society
    | 1 || Sept 12 || [[Dual-Use Research (Biotechnology and Society 2016)|Dual-Use
    2 KB (258 words) - 20:33, 28 November 2016
  • Recombinant DNA Technology (Biotechnology and Society 2016)
    ...ant DNA molecule. You can skim the paper. The main result is shown in Fig. 1.
    2 KB (358 words) - 16:24, 29 August 2016
  • Do-It-Yourself (DIY) Biology versus Bioterrorism (Biotechnology and Society 2016)
    === 1. DIY/Biohacking Czar === ...public participation in the life sciences today. ''J. Sci. Comm.'' '''9''':1-8. [http://jcom.sissa.it/archive/09/01/Jcom0901(2010)C01/Jcom0901(2010)C03
    5 KB (662 words) - 14:39, 11 September 2016
  • Lab Made versus GM Food (Biotechnology and Society 2016)
    == 1. In vitro Meat == #PETA Offers $1 Million Reward to First Person to Make 'Test Tube' Meat <br> Read post on [
    6 KB (895 words) - 19:04, 26 September 2016
  • Probiotics versus Microbiome Engineering (Biotechnology and Society 2016)
    == 1. Fecal Transplants == ...www.nature.com/news/scientists-swab-c-section-babies-with-mothers-microbes-1.19275] Scientists swab C-section babies with mothers' microbes
    3 KB (423 words) - 19:13, 10 October 2016
  • Biofuels versus Fossil Fuels (Biotechnology and Society 2016)
    == 1. Cellulosic Ethanol == ... ecosystem created by hydraulic fracturing in shales] ''Nat Microbiol'' '''1''':16146.
    5 KB (613 words) - 12:23, 11 October 2016
  • Personalized Medicine: Precision versus Privacy (Biotechnology and Society 2016)
    * [http://www.nature.com/news/the-rise-of-the-narciss-ome-1.10240 The rise of the 'narciss-ome'] Carina Dennis ''Nature'' March 16 2012 == 1. Personalized medicine ==
    4 KB (510 words) - 18:47, 17 October 2016
  • Wolbachia versus Gene Drives for Mosquito Control (Biotechnology and Society 2016)
    ...om/news/us-reviews-plan-to-infect-mosquitoes-with-bacteria-to-stop-disease-1.19967 US reviews plan to infect mosquitoes with bacteria to stop disease] E ... Wolbachia Could Defeat Dengue] Scott O'Neill ''Scientific American'' June 1, 2015.
    4 KB (577 words) - 16:24, 7 November 2016
  • Chemical Synthesis of Life (Biotechnology and Society 2016)
    ....ibiology.org/ibioseminars/evolution-ecology/jack-szostak-part-1.html Part 1: The Origin of Cellular Life on Earth] Jack Szostak ''iBiology Lecture'' Ju
    3 KB (347 words) - 14:45, 28 November 2016
  • Human Enhancement versus Cyborgs (Biotechnology and Society 2016)
    ...-Connection/ The Rainbow Connection] Kerry Grens ''The Scientist'' October 1, 2014.
    2 KB (281 words) - 23:03, 28 November 2016
  • IGEM Wiki
    = Heading 1 = <h1>Heading 1</h1>
    5 KB (911 words) - 14:52, 10 June 2019

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